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Inhibition of GDP-[3H]Man transport
| Compound added | % Transport ± SE
|
|
|---|---|---|
| Gmt1 | Gmt2 | |
| None | 100a | 100 |
| GDPb | 12 ± 3 | 13 ± 4 |
| GDP-Man | 19 ± 5 | 19 ± 5 |
| GDP-fucose | 83 ± 13 | 94 ± 16 |
All values were normalized to assays with no added inhibitor (100%). Average values and standard errors shown were calculated from the median values of six independent experiments.
Nonradioactive compounds were present at a final concentration of 23 μM, a 230-fold molar excess over radiolabeled substrate.
