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MUC5AC production in NCI-H292 cells. NCI-H292 cells were cultured in 96-well plates and stimulated with (A) epidermal growth factor (EGF); (B) FXa, thrombin, or EGF; or (C) PAR1-activating peptide (PAR1-AP) or PAR2-AP at the indicated concentrations. MUC5AC production was determined as percentage relative to maximum expression (stimulation with EGF at 1 μg/ml).
