Figure 5.
Phosphorylation of β2AR by various β-agonists. HEK-293 cells were transfected to express FLAG-β2AR and incubated with 32P-orthophosphate as described in Materials and Methods. Cells were treated with the indicated agonists for 10 min at 37°C and purified by immunoprecipitation. (A) Representative autoradiogram. (B) Mean results from five experiments. *P < 0.05 versus Iso. #P < 0.05 versus any other agonist.
