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. 2006 Sep 15;36(2):138–146. doi: 10.1165/rcmb.2006-0253SM

Figure 2.

Figure 2.

NO induces MMP-9 expression in airway epithelial cells. (A) NHBE (solid bars), HBE1 (open bars), or HBE1-NOS2 (shaded bars) cells were grown to 80–90% confluence and treated with either DETA NONOate (10 μM) or l-NMMA for 24 h, after which MMP-9 mRNA was analyzed by semiquantitative RT-PCR and quantitated using densitometry in relation to GADPH expression. (B) Accumulation of secreted MMP-9 protein in the media of HBE1 and HBE1-NOS2 cells was analyzed by gelatin zymography, after 24 h incubation in the absence or presence of DETA NONOate (10 μM) or l-NMMA. (C) Analysis of MMP-9 mRNA expression in HBE1 cells using varying doses of DETA NONOate (10–500 μM). (D) Subconfluent HBE1 (open bars) or HBE1-NOS2 cells (shaded bars) were transiently transfected with a luciferase reporter construct containing the −670 bp MMP-9 promoter sequence, and incubated with either DETA NONOate (10 μM) or l-NMMA for 24 h, after which luciferase activity was measured from cell extracts. Data represent mean ± SE from at least three separate experiments.