Figure 6.

Relative expression (by real-time RT-PCR) of VEGF (A) or CXCL1 (B) in LLC cells implanted with various angiogenic stimuli for 72 h in the flanks of C57B6 mice. Bars represent the mRNA expression of cytokine in LLC cells in sponges relative to LLC cells cultured in vitro in normoxic conditions. In each sponge implanted with the indicated angiogenic stimulus, the expression of the VEGF (A) or CXCL1 (B) was significantly reduced (P < 0.05). (C) VEGF levels (determined by ELISA) in LLC containing sponges co-implanted with CXCL1. In mice lacking CXCR2, VEGF levels are not affected by co-implantation with CXCL1 (P = 0.014 by ANOVA).