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. 2006 Nov 10;36(4):480–490. doi: 10.1165/rcmb.2006-0106OC

Figure 1.

Figure 1.

Figure 1.

Effect of CSE on MMP-2 production and activation in NL9 culture media. Fibroblasts were incubated in the absence (CTL) or in the presence of CSE for the indicated concentrations and times, then the concentrated media were subjected to Western blot analysis using an anti–MMP-2 monoclonal antibody (A and B) or gelatin zymography (C). (A) Dose–response of MMP-2 production in NL9 culture media after 24 h of CSE exposure. (B) Time course of MMP-2 production in NL9 culture media treated with 20% CSE. (C) Gelatin zymography of concentrated NL9 media treated with 20% CSE to measure the induction of MMP-2 activity. The active form of MMP-2 was used as a positive control. Quantitation of Western blot immunoblots were performed by densitometric scanning of blots (A, B, and C, bottom). *P < 0.05.