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. 2006 Nov 10;36(4):480–490. doi: 10.1165/rcmb.2006-0106OC

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Figure 3. — The siRNA duplexes for EGR-1 (EGR-1 siRNA) suppress CSE-induced EGR-1 mRNA, protein expression and MMP-2 activity. NL9 cells were transfected with either 100 nM EGR-1 siRNA (si; solid bars) or scrambled EGR-1 siRNA (sc; open bars). After 24 h of transfection, cells were treated with CSE (20%); NL9 cells transfected with EGR-1 siRNA show a significant reduction in (A) EGR-1 mRNA, (B) EGR-1 protein, and (C) MMP-2 mRNA. (D) Gelatin zymography of concentrated media of NL9 cells treated with CSE (20%) for 2 d. Results of densitometry of active MMP-2 bands are shown in the bar graph at bottom of D.

Figure 3. — The siRNA duplexes for EGR-1 (EGR-1 siRNA) suppress CSE-induced EGR-1 mRNA, protein expression and MMP-2 activity. NL9 cells were transfected with either 100 nM EGR-1 siRNA (si; solid bars) or scrambled EGR-1 siRNA (sc; open bars). After 24 h of transfection, cells were treated with CSE (20%); NL9 cells transfected with EGR-1 siRNA show a significant reduction in (A) EGR-1 mRNA, (B) EGR-1 protein, and (C) MMP-2 mRNA. (D) Gelatin zymography of concentrated media of NL9 cells treated with CSE (20%) for 2 d. Results of densitometry of active MMP-2 bands are shown in the bar graph at bottom of D.