Figure 5.
(A) Chelation of iron in soluble CAPs (sCAPs) using chelex beads substantially reversed CAPs-mediated inhibition of J774 phagocytosis of S. pneumoniae. sCAPs+ H2O, soluble CAPs with chelex-treated sterile deionized distilled H2O; sCAPs+chelex, soluble CAPs were treated with chelex beads. All values are mean ± SD for six separate experiments, each performed in duplicate. **P < 0.01 versus control group. (B) The antioxidant DMTU partially reversed CAPs mediated J774 inhibition of phagocytosis of S. pneumoniae. DMTU: 1 mM/ml. All values represent mean ± SD (n = 3). ++P < 0.01 versus control (C); **P < 0.01 versus CAPs group.