Figure 5.

QRT-PCR validation of differential gene expression in E11.5 lung buds treated with NPAS1-antisense versus -sense ODN. The results given are the pooled data from three separate experiments using three independent RNA preparations from E11.5 lung buds treated with ODN for 48 h. Results are presented as mean percentage change ± SE, comparing npas1-AS–treated lungs versus npas1-sense–treated lungs. For comparison, β-actin is defined as 100% for npas1-AS relative to sense. Significance levels are: *** P < 0.0001; ** P < 0.005; * P < 0.01; ^†† < 0.02; ^† P < 0.05.