Figure 2.

Expression of FAS and surfactant proteins in cells cultured on Matrigel. Type II cells were cultured on Matrigel and harvested 6 d after the addition of the additives as described in Materials and Methods. A shows mRNA values for FAS, SP-A, SP-B, and SP-C normalized to the constitutive probe 36B4 by quantitative real-time PCR for six independent experiments. B shows protein levels by immunoblotting. Lane 1, 1% CS-FBS; lane 2, 1% CS-FBS + K; lane 3, 1% CS-FBS + K + Dex; lane 4, 1% CS-FBS + KIAD. These results are representative of four independent experiments.