TABLE 2.
ACETATE INCORPORATION IN CULTURED HUMAN TYPE II CELLS
| 1% CS-FBS | 1% CS-FBS+KIAD | 5% FBS | 5% FBS+KIAD | |
|---|---|---|---|---|
| μg DNA / well | 4.0 ± 0.1 | 7.2 ± 0.4* | 5.7 ± 0.2 | 7.8 ± 0.2* |
| Acetate incorporation, (cpm × 10−3/μg DNA) | 35.2 ± 9.0 | 170 ± 33.7* | 126.3 ± 37.1 | 298.7 ± 77.3 |
| % total lipids | ||||
| Phospholipids | 67.8 ± 2.1 | 82.8 ± 1.9* | 79.9 ± 4.5 | 85.6 ± 1.6 |
| Neutral lipids | 32.2 ± 2.1 | 17.2 ± 1.9* | 20.1 ± 4.5 | 14.5 ± 1.6 |
| % phospholipids | ||||
| Lyso PC | 1.1 ± 0.2 | 0.5 ± 0.1* | 1.0 ± 0.2 | 0.5 ± 0.1 |
| SM | 8.0 ± 0.7 | 3.7 ± 0.4* | 6.4 ± 1.0 | 2.9 ± 0.4* |
| PC | 75.7 ± 1.6 | 82.6 ± 1.1 | 77.6 ± 1.5 | 82.7 ± 1.1 |
| PS | 2.3 ± 0.4 | 1.8 ± 0.4 | 2.2 ± 0.4 | 1.5 ± 0.2 |
| PI | 5.5 ± 0.6 | 5.6 ± 0.5 | 5.2 ± 0.2 | 6.9 ± 1.0 |
| PE | 6.9 ± 0.4 | 5.5 ± 0.5 | 7.0 ± 0.3 | 4.9 ± 0.5 |
| PG | 0.6 ± 0.1 | 0.3 ± 0.0 | 0.5 ± 0.1 | 0.6 ± 0.1 |
Definition of abbreviations: CS-FBS, charcoal-stripped FBS; FBS, fetal bovine serum; KIAD, keratinocyte growth factor + 3-isobutyl-1-methylxanthine + cyclic AMP + dexamethasone; LysoPC, lysophosphatidylcholine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin.
Type II cells were cultured in the apical access system with the specified media for 6 d. Incorporation of (1-14C) acetate was measured over the last 4 h. The data are expressed at cpm/μg DNA or as percent incorporation into total lipids or individual phospholipids. The results represent the mean ± SE for four independent experiments. The phospholipids measured were LysoPC, SM, PC, PS, PI, PE, and PG.
Indicates there is significant difference between with and without KIAD (P < 0.05).