Figure 4.

BAL fluid proinflammatory cytokines in CFTR-KO mice treated with parthenolide (open bar) or placebo (solid bar). Mice were pretreated with parthenolide 3 μg/g body weight or DMSO alone (placebo) 1 h before intratracheal LPS challenge. BAL was performed at the indicated time. Values represent the mean ± SEM of the concentration (pg/ml) of TNF-α, MIP2, and KC in BAL fluid 1, 3, and 8 h after LPS (n = 6–11 mice in each group). Parthenolide-treated mice had significantly more TNF-α (A) (P = 0.03), MIP2 (B) (P = 0.04), and KC (C) (P = 0.05) at 3 h after LPS as compared with placebo group.