Figure 5.

Effects of in vivo treatment with parthenolide on IκBα degradation in the CF lung. Mice were pretreated with parthenolide 3 μg/g body weight or placebo 1 h before intratracheal LPS challenge, then underwent BAL and the lungs were perfused and removed at the indicated times. (A) Representative autoradiograph of an immunoblot for IκBα. (B) Summary graph of data for degradation of IκBα determined by image analysis of densitometry from autoradiographs. Data show mean ± SEM of scan area of IκBα expression (n = 3 mice in each time point and group) corrected for scan area of β-actin in the same gel lane. Solid bar, placebo; open bar, parthenolide-treated mice.