TABLE 1.
SEQUENCES OF THE OLIGONUCLEOTIDES USED FOR PCR TO PRODUCE 5′- TO 3′ SERIAL TRUNCATION OF hC2GnT-M PROMOTER-LUCIFERASE CONSTRUCTS
| Primer Sequences | Orientation | Product Regions |
|---|---|---|
| 5-tagatctAATCTCTTTTCTCTTCCACTCCAG-3 | F | −417/+187 |
| 5-tgaattagaTCTAGAGCAAGGGGGAACTTTGAG-3 | F | −364/+187 |
| 5-tgaattagaTCTAAGGAGGAACCTACAGGCTGTC-3 | F | −321/+187 |
| 5-tgaattagaTCTGATTAGAAACCAGTTTGATCAC-3 | F | −249/+187 |
| 5- tgaattagaTCTAGAACATTTATTTAATGTTAGA-3 | F | −158/+187 |
| 5-tgaattagaTCTAGCTCCAGCCTAATAAGTAACAC-3 | F | −72/+187 |
| 5-tgaattagaTCTGGGTTTTCCTATTTATCTATCC-3 | F | −42/+187 |
| 5- tgaattagaTCTCTCGCATTACTTCTCTGAGTCA-3 | F | −19/+187 |
| 5- tgaattagaTCTAAGTCACGGGAACTGCCCTTG-3 | F | +18/+187 |
| 5-tgaagtaagcttGTCAGCTTGGTCTTCTCATTGAC-3 | R |
Definition of abbreviations: F, forward primer; R, reverse primer.
Bgl II (AGATCT) and Hind III (AAGCTT) restriction sites added at the 5′ end of the primers used for subcloning are underlined. Ranges of the products relative to the transcription initiation site are indicated.