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. 2007 Mar 15;37(1):67–74. doi: 10.1165/rcmb.2006-0469OC

Figure 6.

Figure 6.

Elevated TGF-β and IL-12p40 levels in Pearl mice after bleomycin challenge. (A) TGF-β1 levels in the lungs of Pearl mice at baseline and after bleomycin challenge. Mice were challenged with intratracheal bleomycin (0.025 U). After 0, 1, 3, or 7 d, lungs were harvested and homogenized. Acid-activated TGF-β1 was measured by ELISA (n = 4 per group, *P < 0.05 for WT versus Pearl at Day 3, **P < 0.001 for WT versus Pearl at Day 7). (B) IL-12/23p40 levels in the lungs of Pearl mice at baseline and after bleomycin challenge. Mice were challenged with intratracheal bleomycin (0.025 U) and levels of IL-12/23p40 in lung homogenate were measured by ELISA (n = 4 per group, *P < 0.05 for WT versus Pearl at all time points). (C) TGF-β1 production from AM after bleomycin challenge in vivo. AMs were isolated from Pearl, Pale Ear, and WT mice at baseline and 24 h after bleomycin challenge. Acid-activated TGF-β1 was measured from the cell media supernatant after 24 h in culture (n = 5 per group for WT, n = 3 per group for Pearl and Pale Ear, *P < 0.05 for Pearl and Pale Ear versus WT). (D) IL-12/23p40 production from AMs after bleomycin challenge in vivo. AMs were isolated from Pearl, Pale Ear, and WT mice at baseline and 24 h after bleomycin challenge. IL-12/23p40 was measured from the cell media supernatant after 24 h in culture (n = 5 per group for WT, n = 3 per group for Pearl and Pale Ear, *P < 0.05 for Pearl and Pale Ear versus WT). Open bars, WT; solid bars, Pearl; shaded bars, Pale Ear.