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. 2007 Mar 8;37(1):97–104. doi: 10.1165/rcmb.2006-0419OC

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Figure 4. — Significant donor variation in NO flux and relative nitrate/nitrite content in culture medium correlates with iNOS gene and protein expression. Cells from different donors show significantly different response to IL-13 as measured by the maximum NO flux after exposure (A), iNOS gene (B), and protein (C) expression. Donor 3 cells are most responsive to increasing IL-13 concentrations, while donor 2 cells are least responsive (n = 6 or 12; #: P < 0.001 compared with donor 2, &: P < 0.01 compared with donor 1). Individual amounts of nitrite ) and nitrate ) in the culture medium were determined after 48 h exposure to IL-13, and the fractional nitrate content [ / + ) ] was calculated. Fractional content was close to 0.5 for all donors under basal conditions (0 ng/ml) and increased concomitant with the IL-13–induced upregulation in iNOS mRNA and protein (D) (n = 6, 13, or 14; *: P < 0.02 compared with 0 ng/ml, $: P < 0.0001 compared with 1 ng/ml).

Inline graphic — Significant donor variation in NO flux and relative nitrate/nitrite content in culture medium correlates with iNOS gene and protein expression. Cells from different donors show significantly different response to IL-13 as measured by the maximum NO flux after exposure (A), iNOS gene (B), and protein (C) expression. Donor 3 cells are most responsive to increasing IL-13 concentrations, while donor 2 cells are least responsive (n = 6 or 12; #: P < 0.001 compared with donor 2, &: P < 0.01 compared with donor 1). Individual amounts of nitrite ) and nitrate ) in the culture medium were determined after 48 h exposure to IL-13, and the fractional nitrate content [ / + ) ] was calculated. Fractional content was close to 0.5 for all donors under basal conditions (0 ng/ml) and increased concomitant with the IL-13–induced upregulation in iNOS mRNA and protein (D) (n = 6, 13, or 14; *: P < 0.02 compared with 0 ng/ml, $: P < 0.0001 compared with 1 ng/ml).