FIG. 1.

RR06 is required for transcription of spr1996 and cbpA. A. Schematic illustration of the rr06-cbpA locus in the chromosome of strain R6 (accession no. AE008564). Open arrows represent the gene directions. The name and length (in bp) of each gene are indicated at the top. The numbers of nucleotides in the intergenic regions are marked below the vertical arrows. Two cbpA transcripts as detected in panel B are illustrated with the thick lines. B. Northern hybridization of strain R6 and its isogenic mutants using the cbpA probe. Total RNA extractions were separated by agarose electrophoresis, blotted, and hybridized with the radiolabeled cbpA probe spanning the 1,065-bp coding sequence at the 5′ region cbpA. The sizes of the RNA molecular mass markers are indicated in kilonucleotides. An ethidium bromide-stained 23S rRNA band in an agarose gel is included to indicate equal loading of the total bacterial RNA. C. Same experiment as in panel B, except for the use of the spr1996 probe, which represents a 446-bp coding sequence of spr1996.