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RR06 is required for expression of the CbpA protein. A. Whole-cell lysates of strain R6 and isogenic mutants in cbpA, rr06, and hk06 (approximately 5 μg total protein/lane) were subjected to SDS-PAGE. The blot was reacted in a Western blot assay with a CbpA rabbit antiserum. The sizes of the protein mass markers are marked in kDa. B. Western blot of strain D39 and its isogenic mutants in rr06 and hk06, as described for panel A.
