FIG. 6.

RR06 is required for pneumococcal adhesion. A. Expression of CbpA at the cell surface of S. pneumoniae was detected by immunofluorescence microscopy. Intact organisms of strains R6 and its isogenic mutants in cbpA, rr06, and hk06 were visualized under phase-contrast (top panel) or fluorescence (bottom panel) microscopy after being stained with the CbpA antiserum. B. Adherence of R6 and its isogenic mutants to Detroit 562 cells. The results of representative experiments are presented as means ± standard deviations of four duplicate wells. C. Same experiment as in panel B, except for the use of A549 cells.