FIG. 7.

RR06 is required for CbpA expression in additional isolates of S. pneumoniae. A. Alignment of the 5′ untranslated sequences of cbpA variants. The nucleotide sequences between the stop codon of spr1996 and the start codon of cbpA from strains R6 (accession no. AE008564), G54 (accession no. AL449923), ST556 (accession no. DQ851866), and TIGR4 (accession no. AE007507) were aligned as described for Fig. 3A. The total number of nucleotides in the cbpA intergenic region for each allele is indicated at the end of each sequence. B. Expression of CbpA in the wild-type ST556 and isogenic rr06 and hk06 mutants. CbpA was detected by Western blotting using purified human complement factor H. C. Same experiment as in panel B, except for the use of cellular lysate from strain G54.