FIG. 2.

Isolation and characterization of new genes encoding dockerin-containing proteins. Genes encoding Cel9P (P90) (A), Gal27A (B), and Xyn10A (P41a) (C) were amplified from the total genomic DNA of C. cellulolyticum by Trap-Dock PCR using convergent inosine-containing degenerate primers. A nonspecific fragment (length, 0.4 kb) was amplified with oligonucleotides p41dir and dock-In (D). Chromosomal DNA walking was performed to clone DNA flanking regions of each gene. S, signal sequence; GH, glycoside hydrolase; D, dockerin domain; X, domain with unknown function. For glycoside hydrolases and CBMs, the number indicates the family.