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. 2007 Jan 12;189(6):2319–2330. doi: 10.1128/JB.01569-06

FIG. 4.

FIG. 4.

Overexpression of rbmB affects colony morphology and biofilm structures. (A) Colony morphologies of the rugose variant and RΔrbmB harboring the overexpression plasmid prbmB-myc or the vector pBAD/Myc-His B grown in the presence of arabinose. (B) CLSM images of the top-down views (large panels) and orthogonal views (side panels) of biofilms formed by RΔrbmB harboring prbmB-myc or the vector in a flow cell system after 24 h postinduction. Mature biofilms were allowed to form in the flow cell chambers in the absence of arabinose for 24 h, followed by overexpression of rbmB with the addition of 0.1% (wt/vol) arabinose to the growth medium. Bars, 40 μm.