FIG. 6.
RbmC and Bap1 are involved in pellicle and biofilm formation. (A) Pellicle formation by the rugose variant, RΔrbmC, RΔbap1, RΔrbmC Δbap1, RΔrbmC Δbap1 harboring prbmC, RΔrbmC Δbap1 harboring pbap1, and RΔrbmC Δbap1 harboring the vector pACYC177 after 2 days of incubation at 30°C. Top and side views of the culture tubes are showed in the top and bottom panels, respectively. (B) Quantitative comparison of biofilm formation by the rugose variant, RΔrbmC, RΔbap1, and RΔrbmC Δbap1 after 8 h of growth at 30°C under static conditions. Error bars represent standard deviations. (C) Expression of the vpsA::lacZ and vpsL::lacZ fusion genes in the rugose variant, RΔrbmC, RΔbap1, and RΔrbmC Δbap1 grown at 30°C to the mid-exponential growth phase. Error bars represent standard deviations.