TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 (φ80lacΔM15) | Promega |
| CC118 (λ pir) | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λ pir | 26 |
| S17-1 (λ pir) | Tpr SmrrecA thi pro rK− mK+RP4:2-Tc:MuKm Tn7 λ pir | 16 |
| V. cholerae strains | ||
| FY_Vc_2 | O1 El Tor A1552, rugose variant, Rifr | 69 |
| FY_Vc_1040 | RΔrbmB, FY_Vc_2 ΔrbmB, Rifr | This study |
| FY_Vc_686 | RΔrbmC, FY_Vc_2 ΔrbmC, Rifr | This study |
| FY_Vc_690 | RΔrbmD, FY_Vc_2 ΔrbmD, Rifr | This study |
| FY_Vc_742 | RΔrbmEF, FY_Vc_2 ΔrbmEF, Rifr | This study |
| FY_Vc_1367 | RΔbap1, FY_Vc_2 Δbap1, Rifr | This study |
| FY_Vc_1400 | RΔrbmC Δbap1, FY_Vc_1367 ΔrbmC, Rifr | This study |
| FY_Vc_222 | FY_Vc_2 mTn7-GFP, Gmr | 20 |
| FY_Vc_1318 | FY_Vc_1040 mTn7-GFP, Gmr | This study |
| FY_Vc_1321 | FY_Vc_686 mTn7-GFP, Gmr | This study |
| FY_Vc_1387 | FY_Vc_690 mTn7-GFP, Gmr | This study |
| FY_Vc_1324 | FY_Vc_742 mTn7-GFP, Gmr | This study |
| FY_Vc_1392 | FY_Vc_1367 mTn7-GFP, Gmr | This study |
| FY_Vc_1395 | FY_Vc_1400 mTn7-GFP, Gmr | This study |
| Plasmids | ||
| pGP704-sacB28 | pGP704 derivative, mob/oriT sacB, Apr | G. Schoolnik |
| pFY-271 | pGP704-sacB28::ΔrbmB, Apr | This study |
| pFY-272 | pGP704-sacB28::ΔrbmC, Apr | This study |
| pFY-273 | pGP704-sacB28::ΔrbmD, Apr | This study |
| pFY-274 | pGP704-sacB28::ΔrbmEF, Apr | This study |
| pFY-330 | pGP704-sacB28::Δbap1, Apr | This study |
| pACYC177 | Multicopy cloning vector, Apr | New England Biolabs |
| pFY-361 | prbmB, pACYC177::rbmB, Apr | This study |
| pFY-362 | prbmC, pACYC177::rbmC, Apr | This study |
| pFY-363 | prbmD, pACYC177::rbmD, Apr | This study |
| pFY-364 | prbmEF, pACYC177::rbmEF operon, Apr | This study |
| pFY-366 | pbap1, pACYC177::bap1, Apr | This study |
| pBAD/Myc-His B | Arabinose-inducible expression vector with C-terminal Myc epitope and six His codons, Apr | Invitrogen |
| pFY-417 | prbmB-myc, pBAD/Myc-His B::rbmB coding region, Apr | This study |
| pFY-420 | prbmC-myc, pBAD/Myc-His B::rbmC coding region, Apr | This study |
| pFY-429 | pbap1-myc, pBAD/Myc-His B::bap1 coding region, Apr | This study |
| pvpsA::lacZ | pRS415 vpsA promoter, Apr | 13 |
| pvpsL::lacZ | pRS415 vpsL promoter, Apr | 13 |
| pMCM11 | pGP704::mTn7-GFP, Gmr Apr | G. Schoolnik |
| pUX-BF13 | oriR6K helper plasmid, mob/oriT, provides Tn7 transposition function in trans, Apr | 6 |