TABLE 1.

Bacterial strains used in this study

Strain	Relevant features	Source or reference
Escherichia coli strains
XL1-Blue		Stratagene
XL1-Blue/pYJ335	E. coli/S. aureus shuttle vector containing a tetracycline-inducible promoter	23
ANG243	pCL55 in XL1-Blue; S. aureus single-site integration vector	33
ANG284	pitet in XL1-Blue; pCL55 containing a tetracycline-inducible promoter	This study
ANG373	pCL55-ypfP in XL1-Blue; expression of YpfP (SAV1017) under native promoter control	This study
ANG374	pCL55-ypfP/ltaA in XL1-Blue; expression of YpfP (SAV1017) and LtaA (SAV1016) under native promoter control	This study
ANG375	pitet-ltaA in XL1-Blue; expression of LtaA (SAV1016) under tetracycline-inducible promoter control	This study
Staphylococcus aureus strains
Newman	Human clinical isolate	9
ΦΝΞ171-39	Newman with transposon insertion in SAV1017 (ypfP)	2
ΦΝΞ177-2	Newman with transposon insertion in SAV2491 (pgcA)	2
ΦΝΞ1952	Newman with transposon insertion in SAV2500 (gtaB)	2
ANG460	Newman with transposon insertion in SAV1016 (ltaA); transduced from strain ANG359	This study
RN4220	Transformable laboratory strain	29
SEJ1	RN4220Δspa; in-frame deletion in spa coding for protein A	Taeok Bae
ANG359	SEJ1 with transposon insertion in SAV1016 (ltaA); transduced	This study
ANG361	SEJ1 with transposon insertion in COL2559; transduced; wild type for LTA expression used as control strain	This study
ANG370	SEJ1 with transposon insertion in SAV1017 (ypfP); transduced from strain ΦΝΞ171-39	This study
ANG371	SEJ1 with transposon insertion in SAV2491 (pgcA); transduced from strain ΦΝΞ177-2	This study
ANG372	SEJ1 with transposon insertion in SAV2500 (gtaB); transduced from strain ΦΝΞ1952	This study
ANG391	pitet-ltaA in strain ANG359 (ltaA [SAV1016] mutant)	This study
ANG393	pitet in strain ANG359 (ltaA [SAV1016] mutant)	This study
ANG395	pitet in strain ANG361 (wild type for LTA)	This study