FIG. 3.

Arrangement of PTS genes. (A) Schematic shows the putative operon structure, the gene products, and the approximate locations of the Tn5 (inverted triangle) and the primers (inverted arrows) used for both the Campbell insertions and the RT-PCR shown in panel B. Bent arrows indicate putative promoters. (B) RT-PCR, performed as described previously (15), shows that the Campbell insertion allele ptsI::pTMO151 (strain KV2801) is not polar on the downstream crr gene. The parent strain KV1421 is shown as a control. ptsI, primers specific for ptsI; crr, primers specific for crr. Lanes 1, 2, 5, 6, strain KV1421; lanes 3, 4, 7, 8, strain KV2801; lane 9, negative control (distilled water); lane 10, positive control (ESR1 genomic DNA).