FIG. 4.

The ptsHI-crr operon regulates bioluminescence. Cells were grown at 28°C in SWT (3) supplemented with extra NaCl (for a final concentration of 552 mM), and bioluminescence was measured over time. To account for any small differences in growth, specific luminescence (relative light units/ml/optical density unit [OD]) was plotted versus optical density. The experiment was performed with two independent isolates (denoted as solid and dotted lines) of the following strains: ES114 (WT, closed squares), KV2532 (mifB::pTMO125, open diamonds), KV2672 (mifA::pKV217, open squares), KV2801 (ptsI::pTMO151, open circles), KV2850 (crr::pTMO152, open triangles), and KV2826 (mifA::pKV217 ΔmifB, gray squares).