FIG. 2.
Metabolic patterns of P- and N- starved cells. Strain ENZ535 (wt) was inoculated 1:500 (time zero) into MOPS medium with a limited amount of ammonium (□) or Pi (○) containing 40 mM glucose and incubated with aeration for 10 days. The pHs of the culture supernatants were determined (B) and adjusted to pH 7 with KOH, and the concentrations of glucose (A) and acetate (C) were determined. The values for days 4, 6, and 8 of incubation are the means ± standard deviations for four independent cultures, and the values for the other days are values for one culture.