FIG. 4.

Lack of PoxB activity affects the viability of P-starved cells. Strains were inoculated 1:500 (time zero) into P_i-limiting medium containing 0.1 mM P_i and 40 mM glucose and incubated with aeration for 14 days. Parentheses indicate that no CFU were detected when 20-μl portions of the cultures were directly plated. The values are the means ± standard deviations for two independent cultures representative of several determinations. (A) Concentrations of ENZ1565 (ΔpflAB) (⋄), ENZ1586 (appY) (▵), ENZ1755 (ΔphoBR) (□), and ENZ1756 (mgsA) (○). (B) Concentrations of ENZ535 (wt) (○), ENZ1222 (poxB::kan) (□), and ENZ1224 (poxB176::lacZ Cm^r) (▵). The survival curves for the mgsA mutant strain (A) and the wt strain (B) are identical.