FIG. 5.
Glutamate protects P-starved cells. Strain ENZ535 (wt) was inoculated 1:500 (time zero) into Pi-limiting medium (0.1 mM Pi plus 40 mM glucose) that was not supplemented (○) or was supplemented with 30 mM glutamate (GLU) (□), 30 mM aspartate (ASP) (•), 30 mM ornithine (ORN) (▿), 30 mM arginine (ARG) (⋄), or 30 mM lysine (LYS) (▵). Parentheses indicate that no CFU were detected when 20-μl portions of the cultures were directly plated. The values are the means ± standard deviations for two independent cultures representative of several determinations. The final pHs determined on day 21 are indicated on the right.