TABLE 2.
Strain | OD600 | pH | Concn (mM) of:
|
||||
---|---|---|---|---|---|---|---|
Glucose | Pyruvate | Acetate | d-Lactate | Formate | |||
wt | 0.84 | 4.70 | 10.1 ± 0.2 | 0.02 ± 0.01 | 31.4 ± 0.3 | 0.19 ± 0.01 | 0.09 ± 0.02 |
pflB | 0.82 | 4.76 | 9.7 ± 0.3 | 0.01 ± 0.01 | 27.4 ± 0.5 | 0.13 ± 0.01 | 0.06 ± 0.01 |
poxB | 0.91 | 4.76 | 10.6 ± 0.3 | 0.88 ± 0.14 | 13.6 ± 0.1 | 1.07 ± 0.01 | 0.11 ± 0.02 |
The viabilities of wt, pflB, and poxB strains are shown in Fig. 4. On day 14 of incubation, the OD600 and the pH of the cultures and the concentrations in the culture supernatants (adjusted to pH 7) of glucose, pyruvate, acetate, d-lactate, and formate were determined. The concentration data are the means ± standard deviations for two independent cultures.