TABLE 3.

Identification of putative CadC-regulated proteins by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry

Functional category	Proteina	Gene name	Spot no.b	NCBI accession no.
Putative CadC-induced proteins
Cell envelope	Outer membrane porin C	ompC	1	2773087
	Outer membrane protein OmpF	ompF	2	468741
Pyruvate dissimilation	Putative formate acetyltransferase	yfiD	3	16765966
Amino acid biosynthesis	Dihydroxy-acid dehydratase	ilvD	4	16767180
	Dihydroxy-acid dehydratase (isoform)	ilvD	5	16767180
	d-3-Phosphoglycerate dehydrogenase	serA	6	16766363
Regulatory functions	Putative transcriptional regulator	STM4270	7	16767520
Translation	30S ribosomal protein S2	rpsB	8	16763606
Putative CadC-repressed proteins
Glycolysis	6-Phosphofructokinase I	pfkA	9	16422627
	6-Phosphofructokinase II	pfkB	10	16764677
	Fructose-bisphosphate aldolase	fbaB	11	16765470
	Putative NAD-dependent aldehyde hydrogenase	STM4519	12	16767763
Tricarboxylic acid cycle	Succinyl-coenzyme A synthetase alpha subunit	sucD	13	16764109
Energy production	ATP synthase subunit beta	atpD	14	6625703
Chaperones and heat shock proteins	Trigger factor	tig	15	16763828
	Heat shock protein HslU	hslU	16	16422658
Amino acid and cofactor biosynthesis	O-Acetylserine sulfhydrolase A	cysK	17	16765750
	HMP-P kinasec	thiD	18	1842118
Regulatory functions	Transcriptional activator	nhaR	19	16763430
	Transcriptional repressor protein MetJ	metJ	20	16767365
Translation	Elongation factor Tu	tuf	21	96718
Unknown, hypothetical	Putative periplasmic protein	STM3030	22	16766332
	Hypothetical protein		23	37962772

^aProteins were identified using ProFound and MASCOT against the NCBI database.

^bProtein spot numbers as shown in Fig. 4.

^cHydroxy-phosphomethylpyrimidine kinase.