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. 2007 Jan 5;189(6):2435–2442. doi: 10.1128/JB.01600-06

FIG. 1.

FIG. 1.

Porin-dependent phosphatase activity of M. smegmatis. (A) The phosphatase activity of M. smegmatis grown in liquid culture was determined using pNPP. Black and gray bars indicate the phosphatase activity in whole and lysed cells, respectively. The inset shows the endogenous phosphatase activity in the parent strains SMR5, MN01, and ML10. Error bars indicate standard deviations. (B) Phosphatase activity of M. smegmatis on Middlebrook 7H10 agar plates containing Tween 80 and BCIP. After 5 days of incubation, pictures of colonies were taken with a Zeiss stereomicroscope Stemi 2000-C using the same magnification for all strains. In both experiments, the strains were SMR5 (wild-type) and the porin mutants MN01 (ΔmspA) and ML10 (ΔmspA ΔmspC), each of which was transformed with the control plasmid pMS2 (control) and the phoA expression plasmid pML440 (pML440+phoA), respectively. The blue color indicates the cleavage of BCIP by PhoA.