FIG. 4.

Expression of phoA in M. bovis BCG. M. bovis BCG strains containing the control plasmid pMS2, the phoA expression plasmid pML440, or a phoA expression cassette integrated at the attachment site of phage L5 were grown in liquid culture. The phosphatase activity in these strains was determined by using pNPP. Black and gray bars indicate the phosphatase activity of whole and lysed cells, respectively. The inset shows the endogenous phosphatase activity of M. bovis BCG. Error bars indicate standard deviations.