TABLE 1.

Bacterial strains and plasmids used in this study^a

Strain or plasmid	Relevant genotype or description	Source or reference
Strains
E. coli DH5α	recA1 endA1 gyrA96 thi relA1 hsdR17(rK− mK+) supE44 φ80ΔlacZΔM15 ΔlacZYA-argF UE169	36
M. smegmatis SMR5	mc2155 derivative, not characterized, high transformation efficiency, Smr (rpsL*)	37
M. smegmatis MN01	SMR5 derivative mspA::Gmr	43
M. smegmatis ML10	SMR5 derivative ΔmspA ΔmspC	45
M. smegmatis ML40	mc2155 derivative, attB::pML443, Hygr	This study
M. smegmatis ML41	ML10 derivative, attB::pML443, Hygr	This study
M. smegmatis ML42	ML10 derivative, attB::pML443, Δhyg	This study
M. smegmatis ML43	MN01 derivative, attB::pML443, Hygr	This study
M. bovis BCG	Pasteur 35739	ATCC
M. bovis BCG ML44	M. bovis BCG derivative, attB::pML443	This study
Plasmids
pMV361	oriE aph attP int	47
pMS2	PAL5000 origin, ColE1 origin, hyg	16
pMN402	PAL5000 origin, ColE1 origin, hyg, phsp60-gfp+	40
pMN234	PAL5000 origin, pBR322 origin, aph, rpsL	46
pML102	PAL5000 origin, ColE1 origin, aph, sacB, int	This study
pML113	ColE1 origin, bla, FRT-hyg-FRT, attP	This study
pML118	ColE1 origin, bla, FRT-hyg-FRT, attP, phsp60-gfp+	This study
pMN252	ColE1 origin, bla, FRT-hyg-FRT, rpsL	46
pML440	PAL5000 origin, ColE1 origin, hyg, pimyc-phoA	This study
pML443	ColE1 origin, bla, FRT-hyg-FRT, attP, pimyc-phoA	This study

^a*, gene encoding a mutated ribosomal protein S12 (K43R) which confers streptomycin resistance (Sm^r).