TABLE 3.
Physiological effects of S. putrefaciens CN-32 with cymA heme variants on the reduction of arsenatea
| Strain | Time (h) | Arsenate (mM) | Arsenite (mM) |
|---|---|---|---|
| All | 0 | 9.9 ± 0.22 | 0.23 ± 0.32 |
| CN-32 + pBBR1MCS-2 | 12.5 | 0.7 ± 0.11 | 9.7 ± 0.00 |
| 50.5 | 0 | 9.7 ± 0.06 | |
| CN-CYMA + pBBR1MCS-2 | 12.5 | 9.7 ± 0.14 | 0.12 ± 0.16 |
| 50.5 | 1.4 ± 0.21 | 8.6 ± 0.01 | |
| CN-CYMA + pH 1(C46S) | 12.5 | 9.2 ± 0.30 | 1.0 ± 0.27 |
| 27.5 | 0 | 10.2 ± 0.50 | |
| 50.5 | 0 | 9.8 ± 0.26 | |
| CN-CYMA + pH 2(C78S) | 12.5 | 10.1 ± 0.12 | 0.10 ± 0.12 |
| 50.5 | 1.5 ± 0.21 | 8.1 ± 0.13 | |
| CN-CYMA + pH 3(C136S) | 12.5 | 9.8 ± 0.03 | 0.18 ± 0.16 |
| 50.5 | 2.5 ± 0.36 | 7.2 ± 0.11 | |
| CN-CYMA + pH 4(C173S) | 12.5 | 9.9 ± 0.22 | 0.07 ± 0.05 |
| 50.5 | 1.4 ± 1.98 | 9.9 ± 0.61 |
a
The cultures represented in Fig. 6B were sampled at the indicated times, and the filtrates were analyzed by high-performance liquid chromatography for arsenate and arsenite. The data and errors represent the means and standard deviations of triplicate cultures, respectively.