Figure 3.

Differential inhibition of recombinant cGPK and cAPK by DT-3, DT-2, and PKI^(5–24). The assays contained 1 nM enzyme, 16 μM substrate peptide TQAKRKKSLAMA (13), 1 μM cAMP/cGMP, 70 nM PKI^(5–24), and 200 nM DT-2 or DT-3, as indicated. Kinase activity was determined for 1.5 min at 30°C in a final volume of 100 μl as described (13).