Figure 4.

Inhibition of endogenous cGPK activity in human aortic smooth muscle cells by internalization of exogenous MTS-fusion peptides DT-2 and DT-3. Cells were preincubated with either W45, DT-5, DT-3, DT-6, or DT-2, and then cell extracts were assayed for kinase activity (n = 3–6). Endogenous cAPK activity was blocked with PKI^(5–24). * and + indicate significant differences (P < 0.05, ANOVA followed by Bonferroni post hoc test) from the untreated and W45 treated control groups, respectively.