Table 1.
Inhibition constants (Ki) of synthetic peptides for cAPK and cGPK
| No. | Sequence | cGPKKi, μM | cAPKKi, μM | cAPK/cGPK |
|---|---|---|---|---|
| WW21 | TQAKRKKALAMA* | 7.5 | 750 | 1.0 × 102 |
| W45 | LRKKKKKH | 0.82 ± 0.33 (10) | 559 ± 108 (4) | 6.8 × 102 |
| 9W28 | LRAKKKKH | 14.5 ± 4.9 (3) | ND | — |
| 9W29 | LRKAKKKH | 11.3 ± 2.6 (3) | ND | — |
| 9W30 | LRKKAKKH | 9.9 ± 2.6 (3) | ND | — |
| 9W31 | LRKKKAKH | 5.3 ± 1.9 (3) | ND | — |
| 9W32 | LRKKKKAH | 46.1 ± 5.5 (3) | ND | — |
| W74 | FLLKKKKKKGHK | 1.2 ± 0.16 (6) | ND | — |
| W75 | FLLKKKKKKHHK | 1.2 ± 0.38 (5) | ND | — |
| DT-3 | RQIKIWFQNRRMKWKK-LRKKKKKH | 0.025 ± 0.009 (8) | 493 ± 86 (3) | 1.97 × 104 |
| DT-5 | RQIKIWFQNRRMKWKK | 0.97 ± 0.24 (4) | 107 ± 32 (4) | 1.10 × 102 |
| DT-2 | YGRKKRRQRRRPP-LRKKKKKH | 0.0125 ± 0.003 (5) | 16.5 ± 3.8 (4) | 1.32 × 103 |
| DT-6 | YGRKKRRQRRRPP | 1.1 ± 0.22 (3) | 26 ± 4 (4) | 2.36 × 101 |
The number of experiments for each Ki are given in brackets. ND, not determined.
*
Taken from ref. 13.