TABLE 2.

Oligonucleotides used in this study

Primer name	Sequence	Description
yfhKP1	GGCAAAGCCTGAATGCGCCTTAGCGACCAGGCGGCGCTGGTCAACCGCACCACGCTTATCGATGCCCGGCGCAGCGAAGCAATGACCAACGCGGCGCTGGATGTAGGCTGGAGCTGCTTC	Construction of the QseE isogenic mutant
yfhKP2	TTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTATATGAATATCCTCCTTA	Construction of the QseE isogenic mutant
yfhARedP1	GAAACTGCTTGGCCTGCGCCTGACCAGCGAAGGCTACAGCGTGGTCACGGGTGTAGGCTGGAGCTGCTTC	Construction of the QseF isogenic mutant
yfhARedP2	AATTCTGTCCGGTTGCGCCCCGCCATTCTCGCCGCGTGGGTGACGTTGCCCATATGAATATCCTCCTTA	Construction of the QseF isogenic mutant
QseFFBAD	GGTACCGTGAAAAGCCCGCGCCATCCA	Construction of pNR02
QseFRBAD	AAGCTTATCGTTTGCATCCAGCTCGTGT	Construction of pNR02
YfhKFBAD	GGTACCTATCTGAACTTCCCCTCGGTT	Construction of pNR01
YfhKRBAD	GAATTCCCTTTCGTGTTTTTCGACGACGG	Construction of pNR01
EspFuF	CGCGGATCCCTGTCGGCTCTCTTCTAGAT	espFu activity assay, EMSA, RT-PCR
EspFuR	CGC GGA TCC ATA TTG CGG TTG ACG GTT GG	espFu activity assay
EspFuSEQ	ATA TTG CGG TTG ACG GTT GG	Primer extension, sequencing ladder
QseE RTR	CGC GCC ATG ATC TTC GA	Real-time analysis
QseE RTF	CCC TTC ACC GCC CCT TT	Real-time analysis
EspFuCInv	TGC GGC GAT GTA TAA ATG AC	Primer extension
EspFuBInv	ACA GCC ATT CCT CCT GTG T	EMSA
EspFuCInv	TGC GGC GAT GTA TAA ATG AC	EMSA
EspFuRN	GTTCATTTTGTACTGGCGGC	RT-PCR
EspFuRT2	GCATCCTATTTATTGCTCACGTTA	RT-PCR
EspFuProbeR	CGAGCGCTTAGATGTATTAATGCC	RT-PCR
EspFuProbeF	ATGATTAACAATGTTTCTTCACTTTTTCC	RT-PCR
QseF RT R	CGT AAG CTG CTG CAA ATT ACC A	Real-time analysis
QseF RT F	CGC CCC GCC ATT CTC	Real-time analysis
3a	GAA ATG GTA CAG GCC ACG CGC ACT	RT-PCR
3b	ATC AGC ACA ATC CAT CGC CCG	RT-PCR
6a	CGG GCG ATG GAT TGT GCT GAT	RT-PCR
6b	TAT GTC GCT CTG CCG CCT GGC	RT-PCR