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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: J Virol Methods. 2007 Feb 15;142(1-2):50–58. doi: 10.1016/j.jviromet.2007.01.006

Figure 2. Schematic of flow cytometry technique to measure murine antibody responses to MCMV.

Figure 2

Cells infected with green fluorescent protein labeled murine cytomegalovirus (GFP-MCMV), shown as green above, are detectable by flow cytometry. These GFP-MCMV infected target cells are incubated with sera from previously infected mice, which contains anti-MCMV antibody, along with phycoerythrein labeled anti-murine IgG1 antibody. Cells that co-localize these fluorophores are detectable by dual color flow cytometry, providing a method to detect development anti-MCMV antibodies in mice.