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. 2007 Jul;9(3):358–367. doi: 10.2353/jmoldx.2007.060149

Figure 1.

Figure 1

On-chip amplification of t(4;14) IgH-MMSET hybrid transcripts from patients with different translocation breakpoints. Both PCR product and the size standards (GS500) were fluorescently labeled (VIC). PCR using cDNA was performed in a three-well PCR chip. CE was performed after manually transferring the PCR product to a glass CE chip. Electropherograms were generated in a μTK. The PCR product is indicated by an asterisk. a–c: On-chip PCR of MB4-1 breakpoints (343 bp): cDNA from different patients having MB4-1 breakpoints were amplified on-chip, and a 1:4 dilution of the product was analyzed by microchip CE. d–f: On-chip PCR of MB4-2 breakpoints (438 bp): cDNA from three patients having MB4-2 breakpoints were amplified on-chip, and a 1:4 dilution of the product was analyzed on a μTK. g–j: On-chip PCR of MB4-3 breakpoints (275 bp): cDNA from four patients having MB4-2 breakpoints were amplified on-chip, and a 1:4 dilution of the product was analyzed on a μTK. The on-chip PCR products from reactions included in a though c were also detected at the appropriate size on the ABI3100 capillary analysis system (not shown).