Table 1.
Sensitivity of the On-Chip t(4;14) PCR
| Thermal cycling | Analysis | Trial | 100% | 10% | 1% | 0.1% | 0.05% | 0.01% | 0.005% | 0.001% | 0.0005% | 0.0001% | Negative control |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Conventional thermal cycler | 2% Agarose gel | a | Y | Y | Y | Y | Y | Y | W | N | N | N | N |
| b | Y | Y | Y | Y | Y | Y | Y | Y | N | N | N | ||
| ABI 3100 | a | Y | Y | Y | Y | Y | Y | Y | Y | N | N | N | |
| b | Y | Y | Y | Y | Y | Y | Y | Y | Y | N | N | ||
| On-chip | μTK | a | Y | Y | Y | Y | Y | Y | N | N | ND | ND | N |
| b | Y | Y | Y | Y | N | N | N | N | ND | ND | N |
KMS-18 cell lines, which express t(4;14) hybrid transcripts, were spiked into normal blood samples at the percentages indicated to prepare two independently constructed dilution series (a and b). On-chip PCR was performed in duplicate, using cDNA in a PCR chip followed by transfer to a glass CE chip for CE. The results were compared with the amplification performed by conventional thermal cycling with analysis using a 2% agarose gel or using capillary separation on the ABI3100.
Y, product detected; N, no product detected; W, weak product; ND, not done.