Figure 1.

Coexpression of CD133, CD34, and KDR in human fetal aorta was analyzed on freshly isolated cells after collagenase dissociation of the tissues. Coexpression of markers was measured by activated cell sorting analysis. a: Live freshly digested CD133⁺ cells. b: Percentages of triple-positive cells are reported in the top right panel. Representative panels are of at least three independent experiments. c: Time-course live culture of immunoselected CD133⁺CD34⁺KDR⁺ cells derived from hFA. Cells in HM-GM grown as floating cluster of cells forming spheroids. Magnification, ×10.