Flow cytometric analysis of caudal spermatozoa from 6-month-old normal, ASMKO+/−, and ASMKO−/− mice based on capacitation status. A representative density-dot profile evaluating whether sperm were able to produce nitric oxide (NO) while undergoing phosphatidylserine translocation (Annex V), both required for capacitation. Note intermediate populations A and B in ASMKO+/− mice sperm (68%), which were also found in the majority of wild-type sperm (95.8%). Population C, which is the majority population in ASMKO−/− mice (95.5%), also was found in heterozygous mice (31.8%).