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. 2007 Jun;170(6):2077–2088. doi: 10.2353/ajpath.2007.061002

Figure 6.

Figure 6

Flow cytometric analysis of caudal spermatozoa from 6-month-old normal, ASMKO+/−, and ASMKO−/− mice based on acrosome status. A representative density-dot profile showing sperm that had undergone the acrosome reaction as assessed by PI labeling and peanut agglutinin (PNA) binding (bottom left quadrants), and those with intact acrosome membranes (bottom right quadrant). Note that only 13% of sperm from ASMKO+/− mice had undergone the acrosome reaction, only slightly more than that in normal mice (∼7%).