Domain organization, four immunodominant epitopes for EBA autoantibodies within the NC1 domain of human type VII collagen, and expression of recombinant CMP. A: The deduced 1253-amino acid sequence of the NC1 domain revealed motifs with homology to known adhesive proteins. CMP, cartilage matrix protein; FNIII 1–9, nine fibronectin type III-like repeats; VWF-A, the A domain of von Willebrand factor. Bottom shows the four previously identified immunodominant epitopes recognized by EBA sera. B: Ten percent SDS-PAGE and Coomassie Blue staining of recombinant GST-CMP protein purified from bacteria. The positions of the 50-kd molecular weight marker and the GST-CMP fusion protein are indicated.