Figure 6.

Histological and immunological examination of lesional skin of SKH1 mice injected with anti-CMP EBA antibodies. A: Histological appearance of skin lesions induced by EBA autoantibodies. H&E staining of lesional murine skin (a) showed separation of the epidermis (e) from the dermis (d). No epidermal-dermal separation was detected in mice receiving equivalent amounts of normal human control IgG (b). B: Immunofluorescence analysis of SKH1 mice injected with anti-CMP EBA antibodies. Cryosections of perilesional and lesional skin as well as other organs were labeled with FITC-conjugated goat anti-human IgG. Note the mice injected with anti-CMP EBA antibodies had in situ deposits of human IgG at the BMZ of perilesional and lesional skin (A and B), perilesional and lesional ear lesions (C and D), esophagus (E and F), and oral mucosal (G). In contrast, mice receiving equivalent amounts of flow-through IgG depleted of reactivity to CMP (H) or purified control normal human IgG (I) had no deposits of human IgG. C: Immunofluorescence analysis was performed with FITC-conjugated goat anti-mouse C3 antibody (α-mC3) and FITC-conjugated goat anti-mouse neutrophil antibody (α-PMN). Note linear deposits of murine C3 at the BMZ of perilesional and lesional skin in mice injected with anti-CMP EBA antibodies. Please also note neutrophils in the dermis. In contrast, in mice receiving equivalent amounts of purified control normal human IgG (control IgG) or flow-through IgG depleted of reactivity to CMP (FT-IgG), no murine C3 deposits or neutrophils were detected.