Figure 3.

Leukocyte subset infiltration in the spinal cord of CD11c^−/− mice with EAE is reduced compared with control mice. A: Leukocytes isolated from spinal cords of control (n = 5) and CD11c^−/− mice (n = 5) as described in Materials and Methods were immunostained for CD45. The infiltration of CD45⁺ leukocytes at day 15 after immunization was markedly reduced in CD11c^−/− mice compared with controls. B: Leukocytes isolated from spinal cords of control (n = 5) and CD11c^−/− mice (n = 5) as described in Materials and Methods were immunostained for CD4 and CD8. The results shown are from cells pooled within each group of mice.