Figure 6.

The effects of AT1R antagonist on the staining of nephrin on day 15 after ANA injection. A: The glomerular immunofluorescence staining for nephrin was semiquantitatively graded using the scale described in Materials and Methods, and the representative staining pattern for each grade is shown. The staining of nephrin on day 15 in the vehicle group displayed various patterns, from a linear pattern (grade 3) to a markedly disrupted pattern (grade 0). No grade 0 glomeruli were observed in the AT1R antagonist group. B: The number of grade 1 glomeruli was less in the AT1R antagonist group than in the vehicle group. (The total number of glomeruli of grade 0 and 1 is 20.6 ± 10.0 in vehicle treatment, and is 11.3 ± 3.6 in AT1RA treatment group; P < 0.05).